m dapt  (Selleck Chemicals)

Journal: Journal of Diabetes Research

Article Title: CD36 Regulates PANoptosis in Diabetic Retinopathy via the NOTCH/MAML Pathway

doi: 10.1155/jdr/9324498

Figure Lengend Snippet: NOTCH pathway modulation affects HG‐induced PANoptosis. hRMECs were treated with a NOTCH agonist (Jagged1 peptide) or inhibitor (DAPT) under HG conditions. (A) Cell viability measured by CCK‐8 assay. (B) Caspase‐3/7 activity measured by colorimetric assay. (C) IL‐1 β and IL‐18 levels were quantified by ELISA. (D) Apoptosis rate measured by flow cytometry with quantification. (E) Quantification of Western blot analysis for PANoptosis‐related proteins, including cleaved Caspase‐3, GSDMD‐N, RIPK1, Bax, RIPK3, p‐MLKL, and Bcl‐2. (F) Co‐IP analysis of PANoptosome components (ASC, Caspase‐1, NLRP3, and RIPK3). (G) qPCR analysis of Bax and Bcl-2 mRNA levels. (H, I) IF staining of p‐MLKL (green) with DAPI (blue) and quantification. Scale bar = 150 μ m. Data are presented as mean ± SD ( n = 3). Statistical analysis was performed using one‐way ANOVA followed by Tukey′s post hoc test. ∗ p < 0.01, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns = not significant.

Article Snippet: To modulate NOTCH signaling under HG conditions, cells were cultured in 25.0 mmol/L D‐glucose for 72 h. During the final 24 h, the HG group was supplemented with either 5 μ g/mL Jagged1 peptide (MedChemExpress, United States) to activate the pathway or 10 μ M DAPT (MedChemExpress, United States) to inhibit it.

Techniques: CCK-8 Assay, Activity Assay, Colorimetric Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot, Co-Immunoprecipitation Assay, Staining

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 1. Time schedules for establishing the three groups (9 rats in each group). Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Control

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 3. Typical microscopic pathological images of HE and Masson staining in the lung tissues of rats in each group (n = 9). (a) HE staining (×100). In the control group, pulmonary alveoli, bronchioles and blood vessels were normal, and no changes in inflammation or fibrosis were observed. In the M group, the infiltration of interstitial inflammatory cells in the lung centered on the airway was remarkable, with proliferated fibrous connective tissue and noticeable proliferated fibrous tissue and smooth muscle tissue around the bronchioles and glands. The alveolar septum was widened with occasional multinucleated giant cells (indicated by a black arrow), and loose granuloma was formed. The inflammatory cell infiltration and fibrous connective tissue proliferation along the bronchus in the M+DAPT group were less severe than those in the M group. (b) Comparison of the collagen volume fractions (CVFs) was analyzed at a magnification of 40x using ImageJ software. CVF is the percentage of the blue area of collagen occupied in the total tissue area, and a higher CVF value indicates more severe fibrosis. (c) Masson staining (×100). Normal thin layers of connective tissue were observed under the submucosa of the bronchioles in the Ctrl group. In the M group, fibrosis of the bronchus and surrounding interstitial tissue was noticeable, and fibrosis developed around the granuloma in some areas (indicated by a yellow arrow). Fibrosis in the M+DAPT group was less severe than that in the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Staining, Control, Comparison, Software

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 4. Western blotting analysis of the ligands and receptors in the Notch pathway in the three groups (9 rats in each group). (a): Analysis of key Notch pathway protein levels. (b)-(g): Levels of the receptor and ligand proteins of the Notch signaling pathway. The letter a indicates p < 0.05 for the M group versus the control group, and the letter b indicates p < 0.05 for the M+DAPT group versus the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Western Blot, Control

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 5. The expression of the M1 macrophage marker genes TNF-α and iNOS and the M2 macrophage marker genes Arg-1 and Mrc2 in the three groups (9 rats in each group) according to PCR. The letter a indicates p < 0.05 for the M group versus the control group, and the letter b indicates p < 0.05 for the M+DAPT group versus the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Expressing, Marker, Control

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 6. Th1/Th2 ratio analysis of lung tissue in the three groups (9 rats in each) according to flow cytometry. The letter a indicates p < 0.05 for the M group versus the control group, and the letter b indicates p < 0.05 for the M+DAPT group versus the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Cytometry, Control

Journal: BioMed Research International

Article Title: Hypoxia Enhances Fusion of Oral Squamous Carcinoma Cells and Epithelial Cells Partly via the Epithelial–Mesenchymal Transition of Epithelial Cells

doi: 10.1155/2018/5015203

Figure Lengend Snippet: (a) The expression of EMT-associated indexes including N-cadherin, E-cadherin, Vimentin, and Slug with/without hypoxia or DAPT stimulation. (b) The number of fused cells by artificial cell counting with/without the pretreated of hypoxia or DAPT for 3 days. (c) The fusion rate of the pretreated group and the control group by FACS. (i) Hypoxia (−), DAPT (−); (ii) hypoxia (−), DAPT (+); (iii) hypoxia (+), DAPT (−); (iv) hypoxia (+), DAPT (+). (d) Statistical analysis of fusion rate of the pretreated group and the control group ( ∗ p < 0.05).

Article Snippet: HIOECs were placed under hypoxia (1% O 2 ) or normoxia (5% CO 2 ) for 24 h; EMT was blocked by 5 μ M DAPT (Catalog number T6202, TargetMol, China).

Techniques: Expressing, Cell Counting